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    ATCC cell lines sh sy5y n atcc cat
    Cell Lines Sh Sy5y N Atcc Cat, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 9295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lines+sh+sy5y+n+atcc+cat/SH-SY5Y/pm41592573-300-231-234
    Average 99 stars, based on 9295 article reviews
    cell lines sh sy5y n atcc cat - by Bioz Stars, 2026-09
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    Bicinchoninic Acid Protein Assay:

    Article Title: Tumor-associated macrophages enhance peripheral nerve tumor infiltration and spinal cord repair.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Arg1 Kindly donated from collaborator N/A iNOS, FITC REAfinity Myltenyi Biotec Cat: 130-116-421; RRID: AB_2727525 Bacterial and virus strains pLV[shRNA]-EGFP:T2A:Puro-U6>mSPP1-shRNA VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro-U6>scramble-shRNA VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro- U6>mRictor VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro- U6>mScramble VectorBuilder N/A AAV9-GFP-U6-mRICTOR-shRNA Biolabs N/A AAV9-GFP-U6-scramble-shRNA Biolabs N/A Biological samples Embryonic (E14.5) mouse neural stem cells This paper N/A Dorsal root ganglia This paper N/A IPSCs-derived motor neurons This paper N/A Chemicals, peptides, and recombinant proteins Recombinant SPP1 R&D Stystems Cat: 441-OP-050/CF DMEM/F-12 GlutaMAX Gibco Cat: 31331-028 B27 Gibco Cat: 17504-044 N2 Gibco Cat: 17502-048 Penicillin/Streptomycin Gibco Cat: 15140-122 EGF Peprotech Cat: AF-100-15 bFGF Preprotech Cat: 100-18B IMDM Euroclone Cat: ECB2072L FBS Euroclone Cat: ECS0186L L-glutamine Gibco Cat: 25030081 M-CSF Miltenyi Biotec Cat: 130101704 LPS Sigma Aldrich Cat: L4005-100MG IFN-y Sigma Aldrich Cat: IF002 IL-4 Miltenyi Biotec Cat: 130-093-920 Neurobasal Media Gibco Cat: 21103-049 StemPro Accutase Cell Dissociation Reagent Gibco Cat: A1105-01 Poly-lysine Sigma-Aldrich Cat: P6407 PFA Mondial Cat: FM0622 HBSS 10X Gibco Cat: 14180-046 Collagenase Roche Cat: 10103578001 Trypsin Sigma-Aldrich Cat: T4665 Parecoxib TargetMol Cat: T1780 Celecoxib LKT Laboratories Cat: 169590-42-5 Batimastat Selleck Chemicals Cat: S7155 RPMI Gibco Cat: 21870076 Lysis Buffer 17 R&D Systems Cat: 895943 StemPro-34 medium Thermo Fisher Scientific Cat: 10639011 SCF Peprotech Cat: 300-07-14UG FLT-3 Peprotech Cat: 300-19-10UG IL-3 Peprotech Cat: 200-03-10UG IL-6 Peprotech Cat: 200-06-20UG Rho-associated kinase inhibitor Selleck Chemicals Cat: Y27632 SB431542 Tocris Cat: 1614 LDN193189 Sigma Aldrich Cat: SML0559 Retinoic acid Sigma Aldrich Cat: R2500-25MG (Continued on next page) e2 Immunity 59, 1–20.e1–e16, February 10, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER BDNF Peprotech Cat: 450-02 Laminin Thermo Fisher Scientific Cat: 23017015 GDNF Peprotech Cat: 450-10-10UG CNTF Peprotech Cat: 450-13-20UG DMEM Gibco Cat: 11965-092 GlutaMAX Supplement Gibco Cat: 35050061 Poly-D lysin Merck Cat: P6407-5MG JRAB2011 MedCheExpress Cat: HY-122022 BSA Sigma Aldrich Cat: A7906-10G CXCL12 Sigma Aldrich Cat: SRP3276-10UG Coomassie Brilliant Blue R250 Sigma Aldrich Cat: 1.12553 Seahorse XF DMEM medium Seahorse Bioscience Cat: 103575-100 Hematoxylin Histo-Line Cat: 01HEMH1000 Eosin Histo-Line Cat: 01EOY102500 Eukitt Sigma Aldrich Cat: 03989 Dextran Biotin 10000 MW Lysine Fixable (BDA) Molecular Probes Cat: D1956 Critical commercial assays Alexa Fluor 488 Tyramide SuperBoost Kit Thermo Fisher Cat: B40932 ELLA Mouse Immunoassay (Simple Plex Cartridge Kit) Bio-Techne Cat: SPCKA-MP-007736 Pierce BCA protein assay kit Thermo Fisher Scientific Cat: 23227 Proteome Profiler Human XL Cytokine Array Kit R&D System Cat: ARY022B Fluo-4 Direct Calcium Assay Kit Invitrogen Cat: F10472 Multi Tissue Dissociation Kit Miltenyi Biotec Cat: 130-110-201 Zombie YellowTM Fixable Viability Kit BioLegend Cat: 423103 DirectZOL RNA Miniprep kit (ZymoResearch) Zymo Research Cat: 2050 CytoTune iPS 2.0 Sendai Reprogrammin Kit Thermo Fisher Scientific Cat: A16517 Vectastain ABC-HRP kit Vector Laboratories Cat: PK-4000 GeneJET PCR Purification Kit Thermo Fisher Scientific Cat: K0702 DC Protein Assay Bio-Rad Cat: 5000111 CyQUANT cell proliferation assay kit Thermo Fisher Scientifc Cat: C35007 Transcription Factor Staining Buffer Set Miltenyi Biotec Cat: 130-122-981 Deposited data scRNA Human Glioblastoma This paper https://zenodo.org/record/ 6046299#.YgZ6bpbSKN4 Experimental models: Cell lines SH-SY5Y-N ATCC Cat: CRL-2266 U87 ATCC Cat: HTB-14 MN/MCA1 MN-MCA1 sarcoma cells were kindly gifted by Professor Antonio Sica, Humanitas Clinical and Research Center, IRCSS, Milan, Italy. .. N/A Experimental models: Organisms/strains C57BL6/J Envigo Cat: 057 B6.Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J Jackson Laboratory Cat: 007909 Tg(-3.1neurog1:GFP) sb2 KIT - European Zebrafish Resource Center (EZRC) - Fish Cat: 15205 Oligonucleotides PTPRC Applied Biosystem Cat: Hs04189704_m1 CD14 Applied Biosystem Cat: Hs02621496_s1 CD206 Applied Biosystem Cat: Hs07288635_g1 (Continued on next page) Immunity 59, 1–20.e1–e16, February 10, 2026 e3

    Calcium Assay:

    Article Title: Tumor-associated macrophages enhance peripheral nerve tumor infiltration and spinal cord repair.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Arg1 Kindly donated from collaborator N/A iNOS, FITC REAfinity Myltenyi Biotec Cat: 130-116-421; RRID: AB_2727525 Bacterial and virus strains pLV[shRNA]-EGFP:T2A:Puro-U6>mSPP1-shRNA VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro-U6>scramble-shRNA VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro- U6>mRictor VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro- U6>mScramble VectorBuilder N/A AAV9-GFP-U6-mRICTOR-shRNA Biolabs N/A AAV9-GFP-U6-scramble-shRNA Biolabs N/A Biological samples Embryonic (E14.5) mouse neural stem cells This paper N/A Dorsal root ganglia This paper N/A IPSCs-derived motor neurons This paper N/A Chemicals, peptides, and recombinant proteins Recombinant SPP1 R&D Stystems Cat: 441-OP-050/CF DMEM/F-12 GlutaMAX Gibco Cat: 31331-028 B27 Gibco Cat: 17504-044 N2 Gibco Cat: 17502-048 Penicillin/Streptomycin Gibco Cat: 15140-122 EGF Peprotech Cat: AF-100-15 bFGF Preprotech Cat: 100-18B IMDM Euroclone Cat: ECB2072L FBS Euroclone Cat: ECS0186L L-glutamine Gibco Cat: 25030081 M-CSF Miltenyi Biotec Cat: 130101704 LPS Sigma Aldrich Cat: L4005-100MG IFN-y Sigma Aldrich Cat: IF002 IL-4 Miltenyi Biotec Cat: 130-093-920 Neurobasal Media Gibco Cat: 21103-049 StemPro Accutase Cell Dissociation Reagent Gibco Cat: A1105-01 Poly-lysine Sigma-Aldrich Cat: P6407 PFA Mondial Cat: FM0622 HBSS 10X Gibco Cat: 14180-046 Collagenase Roche Cat: 10103578001 Trypsin Sigma-Aldrich Cat: T4665 Parecoxib TargetMol Cat: T1780 Celecoxib LKT Laboratories Cat: 169590-42-5 Batimastat Selleck Chemicals Cat: S7155 RPMI Gibco Cat: 21870076 Lysis Buffer 17 R&D Systems Cat: 895943 StemPro-34 medium Thermo Fisher Scientific Cat: 10639011 SCF Peprotech Cat: 300-07-14UG FLT-3 Peprotech Cat: 300-19-10UG IL-3 Peprotech Cat: 200-03-10UG IL-6 Peprotech Cat: 200-06-20UG Rho-associated kinase inhibitor Selleck Chemicals Cat: Y27632 SB431542 Tocris Cat: 1614 LDN193189 Sigma Aldrich Cat: SML0559 Retinoic acid Sigma Aldrich Cat: R2500-25MG (Continued on next page) e2 Immunity 59, 1–20.e1–e16, February 10, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER BDNF Peprotech Cat: 450-02 Laminin Thermo Fisher Scientific Cat: 23017015 GDNF Peprotech Cat: 450-10-10UG CNTF Peprotech Cat: 450-13-20UG DMEM Gibco Cat: 11965-092 GlutaMAX Supplement Gibco Cat: 35050061 Poly-D lysin Merck Cat: P6407-5MG JRAB2011 MedCheExpress Cat: HY-122022 BSA Sigma Aldrich Cat: A7906-10G CXCL12 Sigma Aldrich Cat: SRP3276-10UG Coomassie Brilliant Blue R250 Sigma Aldrich Cat: 1.12553 Seahorse XF DMEM medium Seahorse Bioscience Cat: 103575-100 Hematoxylin Histo-Line Cat: 01HEMH1000 Eosin Histo-Line Cat: 01EOY102500 Eukitt Sigma Aldrich Cat: 03989 Dextran Biotin 10000 MW Lysine Fixable (BDA) Molecular Probes Cat: D1956 Critical commercial assays Alexa Fluor 488 Tyramide SuperBoost Kit Thermo Fisher Cat: B40932 ELLA Mouse Immunoassay (Simple Plex Cartridge Kit) Bio-Techne Cat: SPCKA-MP-007736 Pierce BCA protein assay kit Thermo Fisher Scientific Cat: 23227 Proteome Profiler Human XL Cytokine Array Kit R&D System Cat: ARY022B Fluo-4 Direct Calcium Assay Kit Invitrogen Cat: F10472 Multi Tissue Dissociation Kit Miltenyi Biotec Cat: 130-110-201 Zombie YellowTM Fixable Viability Kit BioLegend Cat: 423103 DirectZOL RNA Miniprep kit (ZymoResearch) Zymo Research Cat: 2050 CytoTune iPS 2.0 Sendai Reprogrammin Kit Thermo Fisher Scientific Cat: A16517 Vectastain ABC-HRP kit Vector Laboratories Cat: PK-4000 GeneJET PCR Purification Kit Thermo Fisher Scientific Cat: K0702 DC Protein Assay Bio-Rad Cat: 5000111 CyQUANT cell proliferation assay kit Thermo Fisher Scientifc Cat: C35007 Transcription Factor Staining Buffer Set Miltenyi Biotec Cat: 130-122-981 Deposited data scRNA Human Glioblastoma This paper https://zenodo.org/record/ 6046299#.YgZ6bpbSKN4 Experimental models: Cell lines SH-SY5Y-N ATCC Cat: CRL-2266 U87 ATCC Cat: HTB-14 MN/MCA1 MN-MCA1 sarcoma cells were kindly gifted by Professor Antonio Sica, Humanitas Clinical and Research Center, IRCSS, Milan, Italy. .. N/A Experimental models: Organisms/strains C57BL6/J Envigo Cat: 057 B6.Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J Jackson Laboratory Cat: 007909 Tg(-3.1neurog1:GFP) sb2 KIT - European Zebrafish Resource Center (EZRC) - Fish Cat: 15205 Oligonucleotides PTPRC Applied Biosystem Cat: Hs04189704_m1 CD14 Applied Biosystem Cat: Hs02621496_s1 CD206 Applied Biosystem Cat: Hs07288635_g1 (Continued on next page) Immunity 59, 1–20.e1–e16, February 10, 2026 e3

    Polymerase Chain Reaction:

    Article Title: Tumor-associated macrophages enhance peripheral nerve tumor infiltration and spinal cord repair.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Arg1 Kindly donated from collaborator N/A iNOS, FITC REAfinity Myltenyi Biotec Cat: 130-116-421; RRID: AB_2727525 Bacterial and virus strains pLV[shRNA]-EGFP:T2A:Puro-U6>mSPP1-shRNA VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro-U6>scramble-shRNA VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro- U6>mRictor VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro- U6>mScramble VectorBuilder N/A AAV9-GFP-U6-mRICTOR-shRNA Biolabs N/A AAV9-GFP-U6-scramble-shRNA Biolabs N/A Biological samples Embryonic (E14.5) mouse neural stem cells This paper N/A Dorsal root ganglia This paper N/A IPSCs-derived motor neurons This paper N/A Chemicals, peptides, and recombinant proteins Recombinant SPP1 R&D Stystems Cat: 441-OP-050/CF DMEM/F-12 GlutaMAX Gibco Cat: 31331-028 B27 Gibco Cat: 17504-044 N2 Gibco Cat: 17502-048 Penicillin/Streptomycin Gibco Cat: 15140-122 EGF Peprotech Cat: AF-100-15 bFGF Preprotech Cat: 100-18B IMDM Euroclone Cat: ECB2072L FBS Euroclone Cat: ECS0186L L-glutamine Gibco Cat: 25030081 M-CSF Miltenyi Biotec Cat: 130101704 LPS Sigma Aldrich Cat: L4005-100MG IFN-y Sigma Aldrich Cat: IF002 IL-4 Miltenyi Biotec Cat: 130-093-920 Neurobasal Media Gibco Cat: 21103-049 StemPro Accutase Cell Dissociation Reagent Gibco Cat: A1105-01 Poly-lysine Sigma-Aldrich Cat: P6407 PFA Mondial Cat: FM0622 HBSS 10X Gibco Cat: 14180-046 Collagenase Roche Cat: 10103578001 Trypsin Sigma-Aldrich Cat: T4665 Parecoxib TargetMol Cat: T1780 Celecoxib LKT Laboratories Cat: 169590-42-5 Batimastat Selleck Chemicals Cat: S7155 RPMI Gibco Cat: 21870076 Lysis Buffer 17 R&D Systems Cat: 895943 StemPro-34 medium Thermo Fisher Scientific Cat: 10639011 SCF Peprotech Cat: 300-07-14UG FLT-3 Peprotech Cat: 300-19-10UG IL-3 Peprotech Cat: 200-03-10UG IL-6 Peprotech Cat: 200-06-20UG Rho-associated kinase inhibitor Selleck Chemicals Cat: Y27632 SB431542 Tocris Cat: 1614 LDN193189 Sigma Aldrich Cat: SML0559 Retinoic acid Sigma Aldrich Cat: R2500-25MG (Continued on next page) e2 Immunity 59, 1–20.e1–e16, February 10, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER BDNF Peprotech Cat: 450-02 Laminin Thermo Fisher Scientific Cat: 23017015 GDNF Peprotech Cat: 450-10-10UG CNTF Peprotech Cat: 450-13-20UG DMEM Gibco Cat: 11965-092 GlutaMAX Supplement Gibco Cat: 35050061 Poly-D lysin Merck Cat: P6407-5MG JRAB2011 MedCheExpress Cat: HY-122022 BSA Sigma Aldrich Cat: A7906-10G CXCL12 Sigma Aldrich Cat: SRP3276-10UG Coomassie Brilliant Blue R250 Sigma Aldrich Cat: 1.12553 Seahorse XF DMEM medium Seahorse Bioscience Cat: 103575-100 Hematoxylin Histo-Line Cat: 01HEMH1000 Eosin Histo-Line Cat: 01EOY102500 Eukitt Sigma Aldrich Cat: 03989 Dextran Biotin 10000 MW Lysine Fixable (BDA) Molecular Probes Cat: D1956 Critical commercial assays Alexa Fluor 488 Tyramide SuperBoost Kit Thermo Fisher Cat: B40932 ELLA Mouse Immunoassay (Simple Plex Cartridge Kit) Bio-Techne Cat: SPCKA-MP-007736 Pierce BCA protein assay kit Thermo Fisher Scientific Cat: 23227 Proteome Profiler Human XL Cytokine Array Kit R&D System Cat: ARY022B Fluo-4 Direct Calcium Assay Kit Invitrogen Cat: F10472 Multi Tissue Dissociation Kit Miltenyi Biotec Cat: 130-110-201 Zombie YellowTM Fixable Viability Kit BioLegend Cat: 423103 DirectZOL RNA Miniprep kit (ZymoResearch) Zymo Research Cat: 2050 CytoTune iPS 2.0 Sendai Reprogrammin Kit Thermo Fisher Scientific Cat: A16517 Vectastain ABC-HRP kit Vector Laboratories Cat: PK-4000 GeneJET PCR Purification Kit Thermo Fisher Scientific Cat: K0702 DC Protein Assay Bio-Rad Cat: 5000111 CyQUANT cell proliferation assay kit Thermo Fisher Scientifc Cat: C35007 Transcription Factor Staining Buffer Set Miltenyi Biotec Cat: 130-122-981 Deposited data scRNA Human Glioblastoma This paper https://zenodo.org/record/ 6046299#.YgZ6bpbSKN4 Experimental models: Cell lines SH-SY5Y-N ATCC Cat: CRL-2266 U87 ATCC Cat: HTB-14 MN/MCA1 MN-MCA1 sarcoma cells were kindly gifted by Professor Antonio Sica, Humanitas Clinical and Research Center, IRCSS, Milan, Italy. .. N/A Experimental models: Organisms/strains C57BL6/J Envigo Cat: 057 B6.Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J Jackson Laboratory Cat: 007909 Tg(-3.1neurog1:GFP) sb2 KIT - European Zebrafish Resource Center (EZRC) - Fish Cat: 15205 Oligonucleotides PTPRC Applied Biosystem Cat: Hs04189704_m1 CD14 Applied Biosystem Cat: Hs02621496_s1 CD206 Applied Biosystem Cat: Hs07288635_g1 (Continued on next page) Immunity 59, 1–20.e1–e16, February 10, 2026 e3

    Purification:

    Article Title: Tumor-associated macrophages enhance peripheral nerve tumor infiltration and spinal cord repair.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Arg1 Kindly donated from collaborator N/A iNOS, FITC REAfinity Myltenyi Biotec Cat: 130-116-421; RRID: AB_2727525 Bacterial and virus strains pLV[shRNA]-EGFP:T2A:Puro-U6>mSPP1-shRNA VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro-U6>scramble-shRNA VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro- U6>mRictor VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro- U6>mScramble VectorBuilder N/A AAV9-GFP-U6-mRICTOR-shRNA Biolabs N/A AAV9-GFP-U6-scramble-shRNA Biolabs N/A Biological samples Embryonic (E14.5) mouse neural stem cells This paper N/A Dorsal root ganglia This paper N/A IPSCs-derived motor neurons This paper N/A Chemicals, peptides, and recombinant proteins Recombinant SPP1 R&D Stystems Cat: 441-OP-050/CF DMEM/F-12 GlutaMAX Gibco Cat: 31331-028 B27 Gibco Cat: 17504-044 N2 Gibco Cat: 17502-048 Penicillin/Streptomycin Gibco Cat: 15140-122 EGF Peprotech Cat: AF-100-15 bFGF Preprotech Cat: 100-18B IMDM Euroclone Cat: ECB2072L FBS Euroclone Cat: ECS0186L L-glutamine Gibco Cat: 25030081 M-CSF Miltenyi Biotec Cat: 130101704 LPS Sigma Aldrich Cat: L4005-100MG IFN-y Sigma Aldrich Cat: IF002 IL-4 Miltenyi Biotec Cat: 130-093-920 Neurobasal Media Gibco Cat: 21103-049 StemPro Accutase Cell Dissociation Reagent Gibco Cat: A1105-01 Poly-lysine Sigma-Aldrich Cat: P6407 PFA Mondial Cat: FM0622 HBSS 10X Gibco Cat: 14180-046 Collagenase Roche Cat: 10103578001 Trypsin Sigma-Aldrich Cat: T4665 Parecoxib TargetMol Cat: T1780 Celecoxib LKT Laboratories Cat: 169590-42-5 Batimastat Selleck Chemicals Cat: S7155 RPMI Gibco Cat: 21870076 Lysis Buffer 17 R&D Systems Cat: 895943 StemPro-34 medium Thermo Fisher Scientific Cat: 10639011 SCF Peprotech Cat: 300-07-14UG FLT-3 Peprotech Cat: 300-19-10UG IL-3 Peprotech Cat: 200-03-10UG IL-6 Peprotech Cat: 200-06-20UG Rho-associated kinase inhibitor Selleck Chemicals Cat: Y27632 SB431542 Tocris Cat: 1614 LDN193189 Sigma Aldrich Cat: SML0559 Retinoic acid Sigma Aldrich Cat: R2500-25MG (Continued on next page) e2 Immunity 59, 1–20.e1–e16, February 10, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER BDNF Peprotech Cat: 450-02 Laminin Thermo Fisher Scientific Cat: 23017015 GDNF Peprotech Cat: 450-10-10UG CNTF Peprotech Cat: 450-13-20UG DMEM Gibco Cat: 11965-092 GlutaMAX Supplement Gibco Cat: 35050061 Poly-D lysin Merck Cat: P6407-5MG JRAB2011 MedCheExpress Cat: HY-122022 BSA Sigma Aldrich Cat: A7906-10G CXCL12 Sigma Aldrich Cat: SRP3276-10UG Coomassie Brilliant Blue R250 Sigma Aldrich Cat: 1.12553 Seahorse XF DMEM medium Seahorse Bioscience Cat: 103575-100 Hematoxylin Histo-Line Cat: 01HEMH1000 Eosin Histo-Line Cat: 01EOY102500 Eukitt Sigma Aldrich Cat: 03989 Dextran Biotin 10000 MW Lysine Fixable (BDA) Molecular Probes Cat: D1956 Critical commercial assays Alexa Fluor 488 Tyramide SuperBoost Kit Thermo Fisher Cat: B40932 ELLA Mouse Immunoassay (Simple Plex Cartridge Kit) Bio-Techne Cat: SPCKA-MP-007736 Pierce BCA protein assay kit Thermo Fisher Scientific Cat: 23227 Proteome Profiler Human XL Cytokine Array Kit R&D System Cat: ARY022B Fluo-4 Direct Calcium Assay Kit Invitrogen Cat: F10472 Multi Tissue Dissociation Kit Miltenyi Biotec Cat: 130-110-201 Zombie YellowTM Fixable Viability Kit BioLegend Cat: 423103 DirectZOL RNA Miniprep kit (ZymoResearch) Zymo Research Cat: 2050 CytoTune iPS 2.0 Sendai Reprogrammin Kit Thermo Fisher Scientific Cat: A16517 Vectastain ABC-HRP kit Vector Laboratories Cat: PK-4000 GeneJET PCR Purification Kit Thermo Fisher Scientific Cat: K0702 DC Protein Assay Bio-Rad Cat: 5000111 CyQUANT cell proliferation assay kit Thermo Fisher Scientifc Cat: C35007 Transcription Factor Staining Buffer Set Miltenyi Biotec Cat: 130-122-981 Deposited data scRNA Human Glioblastoma This paper https://zenodo.org/record/ 6046299#.YgZ6bpbSKN4 Experimental models: Cell lines SH-SY5Y-N ATCC Cat: CRL-2266 U87 ATCC Cat: HTB-14 MN/MCA1 MN-MCA1 sarcoma cells were kindly gifted by Professor Antonio Sica, Humanitas Clinical and Research Center, IRCSS, Milan, Italy. .. N/A Experimental models: Organisms/strains C57BL6/J Envigo Cat: 057 B6.Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J Jackson Laboratory Cat: 007909 Tg(-3.1neurog1:GFP) sb2 KIT - European Zebrafish Resource Center (EZRC) - Fish Cat: 15205 Oligonucleotides PTPRC Applied Biosystem Cat: Hs04189704_m1 CD14 Applied Biosystem Cat: Hs02621496_s1 CD206 Applied Biosystem Cat: Hs07288635_g1 (Continued on next page) Immunity 59, 1–20.e1–e16, February 10, 2026 e3

    DC Protein Assay:

    Article Title: Tumor-associated macrophages enhance peripheral nerve tumor infiltration and spinal cord repair.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Arg1 Kindly donated from collaborator N/A iNOS, FITC REAfinity Myltenyi Biotec Cat: 130-116-421; RRID: AB_2727525 Bacterial and virus strains pLV[shRNA]-EGFP:T2A:Puro-U6>mSPP1-shRNA VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro-U6>scramble-shRNA VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro- U6>mRictor VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro- U6>mScramble VectorBuilder N/A AAV9-GFP-U6-mRICTOR-shRNA Biolabs N/A AAV9-GFP-U6-scramble-shRNA Biolabs N/A Biological samples Embryonic (E14.5) mouse neural stem cells This paper N/A Dorsal root ganglia This paper N/A IPSCs-derived motor neurons This paper N/A Chemicals, peptides, and recombinant proteins Recombinant SPP1 R&D Stystems Cat: 441-OP-050/CF DMEM/F-12 GlutaMAX Gibco Cat: 31331-028 B27 Gibco Cat: 17504-044 N2 Gibco Cat: 17502-048 Penicillin/Streptomycin Gibco Cat: 15140-122 EGF Peprotech Cat: AF-100-15 bFGF Preprotech Cat: 100-18B IMDM Euroclone Cat: ECB2072L FBS Euroclone Cat: ECS0186L L-glutamine Gibco Cat: 25030081 M-CSF Miltenyi Biotec Cat: 130101704 LPS Sigma Aldrich Cat: L4005-100MG IFN-y Sigma Aldrich Cat: IF002 IL-4 Miltenyi Biotec Cat: 130-093-920 Neurobasal Media Gibco Cat: 21103-049 StemPro Accutase Cell Dissociation Reagent Gibco Cat: A1105-01 Poly-lysine Sigma-Aldrich Cat: P6407 PFA Mondial Cat: FM0622 HBSS 10X Gibco Cat: 14180-046 Collagenase Roche Cat: 10103578001 Trypsin Sigma-Aldrich Cat: T4665 Parecoxib TargetMol Cat: T1780 Celecoxib LKT Laboratories Cat: 169590-42-5 Batimastat Selleck Chemicals Cat: S7155 RPMI Gibco Cat: 21870076 Lysis Buffer 17 R&D Systems Cat: 895943 StemPro-34 medium Thermo Fisher Scientific Cat: 10639011 SCF Peprotech Cat: 300-07-14UG FLT-3 Peprotech Cat: 300-19-10UG IL-3 Peprotech Cat: 200-03-10UG IL-6 Peprotech Cat: 200-06-20UG Rho-associated kinase inhibitor Selleck Chemicals Cat: Y27632 SB431542 Tocris Cat: 1614 LDN193189 Sigma Aldrich Cat: SML0559 Retinoic acid Sigma Aldrich Cat: R2500-25MG (Continued on next page) e2 Immunity 59, 1–20.e1–e16, February 10, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER BDNF Peprotech Cat: 450-02 Laminin Thermo Fisher Scientific Cat: 23017015 GDNF Peprotech Cat: 450-10-10UG CNTF Peprotech Cat: 450-13-20UG DMEM Gibco Cat: 11965-092 GlutaMAX Supplement Gibco Cat: 35050061 Poly-D lysin Merck Cat: P6407-5MG JRAB2011 MedCheExpress Cat: HY-122022 BSA Sigma Aldrich Cat: A7906-10G CXCL12 Sigma Aldrich Cat: SRP3276-10UG Coomassie Brilliant Blue R250 Sigma Aldrich Cat: 1.12553 Seahorse XF DMEM medium Seahorse Bioscience Cat: 103575-100 Hematoxylin Histo-Line Cat: 01HEMH1000 Eosin Histo-Line Cat: 01EOY102500 Eukitt Sigma Aldrich Cat: 03989 Dextran Biotin 10000 MW Lysine Fixable (BDA) Molecular Probes Cat: D1956 Critical commercial assays Alexa Fluor 488 Tyramide SuperBoost Kit Thermo Fisher Cat: B40932 ELLA Mouse Immunoassay (Simple Plex Cartridge Kit) Bio-Techne Cat: SPCKA-MP-007736 Pierce BCA protein assay kit Thermo Fisher Scientific Cat: 23227 Proteome Profiler Human XL Cytokine Array Kit R&D System Cat: ARY022B Fluo-4 Direct Calcium Assay Kit Invitrogen Cat: F10472 Multi Tissue Dissociation Kit Miltenyi Biotec Cat: 130-110-201 Zombie YellowTM Fixable Viability Kit BioLegend Cat: 423103 DirectZOL RNA Miniprep kit (ZymoResearch) Zymo Research Cat: 2050 CytoTune iPS 2.0 Sendai Reprogrammin Kit Thermo Fisher Scientific Cat: A16517 Vectastain ABC-HRP kit Vector Laboratories Cat: PK-4000 GeneJET PCR Purification Kit Thermo Fisher Scientific Cat: K0702 DC Protein Assay Bio-Rad Cat: 5000111 CyQUANT cell proliferation assay kit Thermo Fisher Scientifc Cat: C35007 Transcription Factor Staining Buffer Set Miltenyi Biotec Cat: 130-122-981 Deposited data scRNA Human Glioblastoma This paper https://zenodo.org/record/ 6046299#.YgZ6bpbSKN4 Experimental models: Cell lines SH-SY5Y-N ATCC Cat: CRL-2266 U87 ATCC Cat: HTB-14 MN/MCA1 MN-MCA1 sarcoma cells were kindly gifted by Professor Antonio Sica, Humanitas Clinical and Research Center, IRCSS, Milan, Italy. .. N/A Experimental models: Organisms/strains C57BL6/J Envigo Cat: 057 B6.Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J Jackson Laboratory Cat: 007909 Tg(-3.1neurog1:GFP) sb2 KIT - European Zebrafish Resource Center (EZRC) - Fish Cat: 15205 Oligonucleotides PTPRC Applied Biosystem Cat: Hs04189704_m1 CD14 Applied Biosystem Cat: Hs02621496_s1 CD206 Applied Biosystem Cat: Hs07288635_g1 (Continued on next page) Immunity 59, 1–20.e1–e16, February 10, 2026 e3

    CyQUANT Assay:

    Article Title: Tumor-associated macrophages enhance peripheral nerve tumor infiltration and spinal cord repair.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Arg1 Kindly donated from collaborator N/A iNOS, FITC REAfinity Myltenyi Biotec Cat: 130-116-421; RRID: AB_2727525 Bacterial and virus strains pLV[shRNA]-EGFP:T2A:Puro-U6>mSPP1-shRNA VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro-U6>scramble-shRNA VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro- U6>mRictor VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro- U6>mScramble VectorBuilder N/A AAV9-GFP-U6-mRICTOR-shRNA Biolabs N/A AAV9-GFP-U6-scramble-shRNA Biolabs N/A Biological samples Embryonic (E14.5) mouse neural stem cells This paper N/A Dorsal root ganglia This paper N/A IPSCs-derived motor neurons This paper N/A Chemicals, peptides, and recombinant proteins Recombinant SPP1 R&D Stystems Cat: 441-OP-050/CF DMEM/F-12 GlutaMAX Gibco Cat: 31331-028 B27 Gibco Cat: 17504-044 N2 Gibco Cat: 17502-048 Penicillin/Streptomycin Gibco Cat: 15140-122 EGF Peprotech Cat: AF-100-15 bFGF Preprotech Cat: 100-18B IMDM Euroclone Cat: ECB2072L FBS Euroclone Cat: ECS0186L L-glutamine Gibco Cat: 25030081 M-CSF Miltenyi Biotec Cat: 130101704 LPS Sigma Aldrich Cat: L4005-100MG IFN-y Sigma Aldrich Cat: IF002 IL-4 Miltenyi Biotec Cat: 130-093-920 Neurobasal Media Gibco Cat: 21103-049 StemPro Accutase Cell Dissociation Reagent Gibco Cat: A1105-01 Poly-lysine Sigma-Aldrich Cat: P6407 PFA Mondial Cat: FM0622 HBSS 10X Gibco Cat: 14180-046 Collagenase Roche Cat: 10103578001 Trypsin Sigma-Aldrich Cat: T4665 Parecoxib TargetMol Cat: T1780 Celecoxib LKT Laboratories Cat: 169590-42-5 Batimastat Selleck Chemicals Cat: S7155 RPMI Gibco Cat: 21870076 Lysis Buffer 17 R&D Systems Cat: 895943 StemPro-34 medium Thermo Fisher Scientific Cat: 10639011 SCF Peprotech Cat: 300-07-14UG FLT-3 Peprotech Cat: 300-19-10UG IL-3 Peprotech Cat: 200-03-10UG IL-6 Peprotech Cat: 200-06-20UG Rho-associated kinase inhibitor Selleck Chemicals Cat: Y27632 SB431542 Tocris Cat: 1614 LDN193189 Sigma Aldrich Cat: SML0559 Retinoic acid Sigma Aldrich Cat: R2500-25MG (Continued on next page) e2 Immunity 59, 1–20.e1–e16, February 10, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER BDNF Peprotech Cat: 450-02 Laminin Thermo Fisher Scientific Cat: 23017015 GDNF Peprotech Cat: 450-10-10UG CNTF Peprotech Cat: 450-13-20UG DMEM Gibco Cat: 11965-092 GlutaMAX Supplement Gibco Cat: 35050061 Poly-D lysin Merck Cat: P6407-5MG JRAB2011 MedCheExpress Cat: HY-122022 BSA Sigma Aldrich Cat: A7906-10G CXCL12 Sigma Aldrich Cat: SRP3276-10UG Coomassie Brilliant Blue R250 Sigma Aldrich Cat: 1.12553 Seahorse XF DMEM medium Seahorse Bioscience Cat: 103575-100 Hematoxylin Histo-Line Cat: 01HEMH1000 Eosin Histo-Line Cat: 01EOY102500 Eukitt Sigma Aldrich Cat: 03989 Dextran Biotin 10000 MW Lysine Fixable (BDA) Molecular Probes Cat: D1956 Critical commercial assays Alexa Fluor 488 Tyramide SuperBoost Kit Thermo Fisher Cat: B40932 ELLA Mouse Immunoassay (Simple Plex Cartridge Kit) Bio-Techne Cat: SPCKA-MP-007736 Pierce BCA protein assay kit Thermo Fisher Scientific Cat: 23227 Proteome Profiler Human XL Cytokine Array Kit R&D System Cat: ARY022B Fluo-4 Direct Calcium Assay Kit Invitrogen Cat: F10472 Multi Tissue Dissociation Kit Miltenyi Biotec Cat: 130-110-201 Zombie YellowTM Fixable Viability Kit BioLegend Cat: 423103 DirectZOL RNA Miniprep kit (ZymoResearch) Zymo Research Cat: 2050 CytoTune iPS 2.0 Sendai Reprogrammin Kit Thermo Fisher Scientific Cat: A16517 Vectastain ABC-HRP kit Vector Laboratories Cat: PK-4000 GeneJET PCR Purification Kit Thermo Fisher Scientific Cat: K0702 DC Protein Assay Bio-Rad Cat: 5000111 CyQUANT cell proliferation assay kit Thermo Fisher Scientifc Cat: C35007 Transcription Factor Staining Buffer Set Miltenyi Biotec Cat: 130-122-981 Deposited data scRNA Human Glioblastoma This paper https://zenodo.org/record/ 6046299#.YgZ6bpbSKN4 Experimental models: Cell lines SH-SY5Y-N ATCC Cat: CRL-2266 U87 ATCC Cat: HTB-14 MN/MCA1 MN-MCA1 sarcoma cells were kindly gifted by Professor Antonio Sica, Humanitas Clinical and Research Center, IRCSS, Milan, Italy. .. N/A Experimental models: Organisms/strains C57BL6/J Envigo Cat: 057 B6.Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J Jackson Laboratory Cat: 007909 Tg(-3.1neurog1:GFP) sb2 KIT - European Zebrafish Resource Center (EZRC) - Fish Cat: 15205 Oligonucleotides PTPRC Applied Biosystem Cat: Hs04189704_m1 CD14 Applied Biosystem Cat: Hs02621496_s1 CD206 Applied Biosystem Cat: Hs07288635_g1 (Continued on next page) Immunity 59, 1–20.e1–e16, February 10, 2026 e3

    Proliferation Assay:

    Article Title: Tumor-associated macrophages enhance peripheral nerve tumor infiltration and spinal cord repair.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Arg1 Kindly donated from collaborator N/A iNOS, FITC REAfinity Myltenyi Biotec Cat: 130-116-421; RRID: AB_2727525 Bacterial and virus strains pLV[shRNA]-EGFP:T2A:Puro-U6>mSPP1-shRNA VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro-U6>scramble-shRNA VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro- U6>mRictor VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro- U6>mScramble VectorBuilder N/A AAV9-GFP-U6-mRICTOR-shRNA Biolabs N/A AAV9-GFP-U6-scramble-shRNA Biolabs N/A Biological samples Embryonic (E14.5) mouse neural stem cells This paper N/A Dorsal root ganglia This paper N/A IPSCs-derived motor neurons This paper N/A Chemicals, peptides, and recombinant proteins Recombinant SPP1 R&D Stystems Cat: 441-OP-050/CF DMEM/F-12 GlutaMAX Gibco Cat: 31331-028 B27 Gibco Cat: 17504-044 N2 Gibco Cat: 17502-048 Penicillin/Streptomycin Gibco Cat: 15140-122 EGF Peprotech Cat: AF-100-15 bFGF Preprotech Cat: 100-18B IMDM Euroclone Cat: ECB2072L FBS Euroclone Cat: ECS0186L L-glutamine Gibco Cat: 25030081 M-CSF Miltenyi Biotec Cat: 130101704 LPS Sigma Aldrich Cat: L4005-100MG IFN-y Sigma Aldrich Cat: IF002 IL-4 Miltenyi Biotec Cat: 130-093-920 Neurobasal Media Gibco Cat: 21103-049 StemPro Accutase Cell Dissociation Reagent Gibco Cat: A1105-01 Poly-lysine Sigma-Aldrich Cat: P6407 PFA Mondial Cat: FM0622 HBSS 10X Gibco Cat: 14180-046 Collagenase Roche Cat: 10103578001 Trypsin Sigma-Aldrich Cat: T4665 Parecoxib TargetMol Cat: T1780 Celecoxib LKT Laboratories Cat: 169590-42-5 Batimastat Selleck Chemicals Cat: S7155 RPMI Gibco Cat: 21870076 Lysis Buffer 17 R&D Systems Cat: 895943 StemPro-34 medium Thermo Fisher Scientific Cat: 10639011 SCF Peprotech Cat: 300-07-14UG FLT-3 Peprotech Cat: 300-19-10UG IL-3 Peprotech Cat: 200-03-10UG IL-6 Peprotech Cat: 200-06-20UG Rho-associated kinase inhibitor Selleck Chemicals Cat: Y27632 SB431542 Tocris Cat: 1614 LDN193189 Sigma Aldrich Cat: SML0559 Retinoic acid Sigma Aldrich Cat: R2500-25MG (Continued on next page) e2 Immunity 59, 1–20.e1–e16, February 10, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER BDNF Peprotech Cat: 450-02 Laminin Thermo Fisher Scientific Cat: 23017015 GDNF Peprotech Cat: 450-10-10UG CNTF Peprotech Cat: 450-13-20UG DMEM Gibco Cat: 11965-092 GlutaMAX Supplement Gibco Cat: 35050061 Poly-D lysin Merck Cat: P6407-5MG JRAB2011 MedCheExpress Cat: HY-122022 BSA Sigma Aldrich Cat: A7906-10G CXCL12 Sigma Aldrich Cat: SRP3276-10UG Coomassie Brilliant Blue R250 Sigma Aldrich Cat: 1.12553 Seahorse XF DMEM medium Seahorse Bioscience Cat: 103575-100 Hematoxylin Histo-Line Cat: 01HEMH1000 Eosin Histo-Line Cat: 01EOY102500 Eukitt Sigma Aldrich Cat: 03989 Dextran Biotin 10000 MW Lysine Fixable (BDA) Molecular Probes Cat: D1956 Critical commercial assays Alexa Fluor 488 Tyramide SuperBoost Kit Thermo Fisher Cat: B40932 ELLA Mouse Immunoassay (Simple Plex Cartridge Kit) Bio-Techne Cat: SPCKA-MP-007736 Pierce BCA protein assay kit Thermo Fisher Scientific Cat: 23227 Proteome Profiler Human XL Cytokine Array Kit R&D System Cat: ARY022B Fluo-4 Direct Calcium Assay Kit Invitrogen Cat: F10472 Multi Tissue Dissociation Kit Miltenyi Biotec Cat: 130-110-201 Zombie YellowTM Fixable Viability Kit BioLegend Cat: 423103 DirectZOL RNA Miniprep kit (ZymoResearch) Zymo Research Cat: 2050 CytoTune iPS 2.0 Sendai Reprogrammin Kit Thermo Fisher Scientific Cat: A16517 Vectastain ABC-HRP kit Vector Laboratories Cat: PK-4000 GeneJET PCR Purification Kit Thermo Fisher Scientific Cat: K0702 DC Protein Assay Bio-Rad Cat: 5000111 CyQUANT cell proliferation assay kit Thermo Fisher Scientifc Cat: C35007 Transcription Factor Staining Buffer Set Miltenyi Biotec Cat: 130-122-981 Deposited data scRNA Human Glioblastoma This paper https://zenodo.org/record/ 6046299#.YgZ6bpbSKN4 Experimental models: Cell lines SH-SY5Y-N ATCC Cat: CRL-2266 U87 ATCC Cat: HTB-14 MN/MCA1 MN-MCA1 sarcoma cells were kindly gifted by Professor Antonio Sica, Humanitas Clinical and Research Center, IRCSS, Milan, Italy. .. N/A Experimental models: Organisms/strains C57BL6/J Envigo Cat: 057 B6.Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J Jackson Laboratory Cat: 007909 Tg(-3.1neurog1:GFP) sb2 KIT - European Zebrafish Resource Center (EZRC) - Fish Cat: 15205 Oligonucleotides PTPRC Applied Biosystem Cat: Hs04189704_m1 CD14 Applied Biosystem Cat: Hs02621496_s1 CD206 Applied Biosystem Cat: Hs07288635_g1 (Continued on next page) Immunity 59, 1–20.e1–e16, February 10, 2026 e3

    Staining:

    Article Title: Tumor-associated macrophages enhance peripheral nerve tumor infiltration and spinal cord repair.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Arg1 Kindly donated from collaborator N/A iNOS, FITC REAfinity Myltenyi Biotec Cat: 130-116-421; RRID: AB_2727525 Bacterial and virus strains pLV[shRNA]-EGFP:T2A:Puro-U6>mSPP1-shRNA VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro-U6>scramble-shRNA VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro- U6>mRictor VectorBuilder N/A pLV[shRNA]-EGFP:T2A:Puro- U6>mScramble VectorBuilder N/A AAV9-GFP-U6-mRICTOR-shRNA Biolabs N/A AAV9-GFP-U6-scramble-shRNA Biolabs N/A Biological samples Embryonic (E14.5) mouse neural stem cells This paper N/A Dorsal root ganglia This paper N/A IPSCs-derived motor neurons This paper N/A Chemicals, peptides, and recombinant proteins Recombinant SPP1 R&D Stystems Cat: 441-OP-050/CF DMEM/F-12 GlutaMAX Gibco Cat: 31331-028 B27 Gibco Cat: 17504-044 N2 Gibco Cat: 17502-048 Penicillin/Streptomycin Gibco Cat: 15140-122 EGF Peprotech Cat: AF-100-15 bFGF Preprotech Cat: 100-18B IMDM Euroclone Cat: ECB2072L FBS Euroclone Cat: ECS0186L L-glutamine Gibco Cat: 25030081 M-CSF Miltenyi Biotec Cat: 130101704 LPS Sigma Aldrich Cat: L4005-100MG IFN-y Sigma Aldrich Cat: IF002 IL-4 Miltenyi Biotec Cat: 130-093-920 Neurobasal Media Gibco Cat: 21103-049 StemPro Accutase Cell Dissociation Reagent Gibco Cat: A1105-01 Poly-lysine Sigma-Aldrich Cat: P6407 PFA Mondial Cat: FM0622 HBSS 10X Gibco Cat: 14180-046 Collagenase Roche Cat: 10103578001 Trypsin Sigma-Aldrich Cat: T4665 Parecoxib TargetMol Cat: T1780 Celecoxib LKT Laboratories Cat: 169590-42-5 Batimastat Selleck Chemicals Cat: S7155 RPMI Gibco Cat: 21870076 Lysis Buffer 17 R&D Systems Cat: 895943 StemPro-34 medium Thermo Fisher Scientific Cat: 10639011 SCF Peprotech Cat: 300-07-14UG FLT-3 Peprotech Cat: 300-19-10UG IL-3 Peprotech Cat: 200-03-10UG IL-6 Peprotech Cat: 200-06-20UG Rho-associated kinase inhibitor Selleck Chemicals Cat: Y27632 SB431542 Tocris Cat: 1614 LDN193189 Sigma Aldrich Cat: SML0559 Retinoic acid Sigma Aldrich Cat: R2500-25MG (Continued on next page) e2 Immunity 59, 1–20.e1–e16, February 10, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER BDNF Peprotech Cat: 450-02 Laminin Thermo Fisher Scientific Cat: 23017015 GDNF Peprotech Cat: 450-10-10UG CNTF Peprotech Cat: 450-13-20UG DMEM Gibco Cat: 11965-092 GlutaMAX Supplement Gibco Cat: 35050061 Poly-D lysin Merck Cat: P6407-5MG JRAB2011 MedCheExpress Cat: HY-122022 BSA Sigma Aldrich Cat: A7906-10G CXCL12 Sigma Aldrich Cat: SRP3276-10UG Coomassie Brilliant Blue R250 Sigma Aldrich Cat: 1.12553 Seahorse XF DMEM medium Seahorse Bioscience Cat: 103575-100 Hematoxylin Histo-Line Cat: 01HEMH1000 Eosin Histo-Line Cat: 01EOY102500 Eukitt Sigma Aldrich Cat: 03989 Dextran Biotin 10000 MW Lysine Fixable (BDA) Molecular Probes Cat: D1956 Critical commercial assays Alexa Fluor 488 Tyramide SuperBoost Kit Thermo Fisher Cat: B40932 ELLA Mouse Immunoassay (Simple Plex Cartridge Kit) Bio-Techne Cat: SPCKA-MP-007736 Pierce BCA protein assay kit Thermo Fisher Scientific Cat: 23227 Proteome Profiler Human XL Cytokine Array Kit R&D System Cat: ARY022B Fluo-4 Direct Calcium Assay Kit Invitrogen Cat: F10472 Multi Tissue Dissociation Kit Miltenyi Biotec Cat: 130-110-201 Zombie YellowTM Fixable Viability Kit BioLegend Cat: 423103 DirectZOL RNA Miniprep kit (ZymoResearch) Zymo Research Cat: 2050 CytoTune iPS 2.0 Sendai Reprogrammin Kit Thermo Fisher Scientific Cat: A16517 Vectastain ABC-HRP kit Vector Laboratories Cat: PK-4000 GeneJET PCR Purification Kit Thermo Fisher Scientific Cat: K0702 DC Protein Assay Bio-Rad Cat: 5000111 CyQUANT cell proliferation assay kit Thermo Fisher Scientifc Cat: C35007 Transcription Factor Staining Buffer Set Miltenyi Biotec Cat: 130-122-981 Deposited data scRNA Human Glioblastoma This paper https://zenodo.org/record/ 6046299#.YgZ6bpbSKN4 Experimental models: Cell lines SH-SY5Y-N ATCC Cat: CRL-2266 U87 ATCC Cat: HTB-14 MN/MCA1 MN-MCA1 sarcoma cells were kindly gifted by Professor Antonio Sica, Humanitas Clinical and Research Center, IRCSS, Milan, Italy. .. N/A Experimental models: Organisms/strains C57BL6/J Envigo Cat: 057 B6.Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J Jackson Laboratory Cat: 007909 Tg(-3.1neurog1:GFP) sb2 KIT - European Zebrafish Resource Center (EZRC) - Fish Cat: 15205 Oligonucleotides PTPRC Applied Biosystem Cat: Hs04189704_m1 CD14 Applied Biosystem Cat: Hs02621496_s1 CD206 Applied Biosystem Cat: Hs07288635_g1 (Continued on next page) Immunity 59, 1–20.e1–e16, February 10, 2026 e3



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